RNA looks like contaminated is working fine during PCR?

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How do I increase the yield of my PCR product?

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Do I need to strip the PVDF membrane before staining it with colloidal gold?

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Research on Supply Chain Management in Indian context?

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PACYC PCR not working after several attempts. Would anyone have any suggestions?

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Craig Silver

It is not possible to obtain repeatable, accurate results from a degraded RNA sample, or an RNA sample that has organic contaminants in it. Best to either re-extract your sample, or you can re-purify via alcohol precipitation.

Md. Mahiuddin Zahangir

Degraded RNA will not give you better result in a continuous manner. and i hope it will not give you good results on qPCR. Its better to re-extract RNA for further works.

Thanks