Hey,
i am looking for a extraction method for RNA (from wheat) without the use of a kit. I tested one successfully but only for sugar beet, maybe someone has a good protocol for wheat RNA extraction or some tips where i can find a protocol.
Thanks!!
I found this paper in the literature; hope will be useful to you.
Thanks,
Ram Singh
There are a variety of kits that may still help with good RNA extraction.
Here is a paper that compares a few Plant RNA Kits for isolation from some challenging woody grapevine samples in this case:
http://bit.ly/1N8G98c
and a poster that compares various kits for RNA extraction from a range of plant types.
http://bit.ly/1TBXo8B
Hope this is helpful
Thanks a lot Ram J Singh, i will have a look in this paper, it seems that they use a similar method i was using before (but for sugar beet)
Thanks also Tom, but i am specially looking for a isolation method without using a kit. But thanks for your links, i will also check them!!
This is an exciting project! We work on vanilla planifolia plantations in North-Eastern Madagascar & we have made many observations of ants on vanilla harvesting extra-floral nectar. Our...
29 January 2018 1,755 3 View
CSU website http://sgedg.weizmann.ac.il/csu. contacts for structural units by authors (Sobolev V., Sorokine A., Prilusky J., Abola E.E. and Edelman M. (1999) Automated analysis of interatomic...
21 June 2017 5,580 5 View
I am trying to identify different morphs of Phytmanus crucifer and other anemone species in Bocas del Toro, Caribbean Panama.
08 April 2014 5,540 2 View
I am looking for evidence on the application/utilization of the Chronic Care Model in geriatric care, in particular in the reactivation trajectory after hospital discharge (e.g. somatic unit, PG...
25 September 2012 4,836 3 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...
10 August 2024 8,539 5 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...
06 August 2024 807 3 View
I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...
06 August 2024 1,367 3 View
I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
05 August 2024 9,798 3 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
HOW CAN I WRITE A CODE TO USE THE WAVENET TRANSFORM AS A FEATURE EXTRACTION METHOD INSTEAD OF DWT IN MATLAB?
03 August 2024 7,829 0 View