Thanx Sebastian......I will starting in situ for the first time...so I needed some suggestions and precautions for that...thanx for ur suggestions...I wud certainly consider them....We will b doing DIG labelling only...but detection is by NBT/BCIP which is for anti-DIG antibody conjugated with AP. That wont make much of a difference....
May I knw whether u r into plant or animal sciences???Also which field r u in???? What sort of work profile????In which system have u done insitu?