Hi all,
I'm not an expert on virology, but I'm trying to look for any RNA extraction methods that are preferably bead-based, for potential integration to a liquid handling robot workflow. I know there are many automation systems from different companies on the market, but I don't want to rely on a package deal that ties everything (reagents, type of beads and the equipment) to a single company.
Any suggestions/papers?
Thanks!
You can use oligoT magnetic beads that will pull down polyA+ RNAArticle Isolation of Poly(A) + Messenger RNA Using Magnetic Oligo(dT) Beads
But if you're isolating viral RNA from cell culture medium, the viruses are typically filtered (0.2um) and centrifuged at high speeds (in my experience) but there are commercial kits that can extract RNA just from bronchial lavage samples, but to do this they likely bind a specific complementary oligonucleotide of whichever viral RNA they are interested in to magnetic beads like Thermofisher's magmax kit.
But if your virus has a polyA tail then oligoT beads should be enough tho you will get mRNA in there too if the viruses aren't purified first.
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