RNA extraction method

More Gül Otlu's questions See All

Hi, has anyone carried out stereotaxic injection to SCN in Nile grassed rat?

I am looking for the coordinates of SCN?

30 July 2023 3,337 0 View

Why is the estimated regression weight p value different from the direct effect p value in the AMOS program?

Hello everyone. The p value of the path estime regression weight (B=0.198) from A to C, is 0.014 in my model in the figure. After boostraping, the coefficient from A to C (B=0.198) becomes p value...

17 June 2023 9,295 3 View

Hi everyone, is it possible to perform SU8-3050 spin coating under 1000 rpm?

Hello, ı want to produce a thick layer of SU8-3050 -more than 100 micrometers-. Instead of a double layer, can ı perform the spin coating under 1000 rpm, does it form a homogenous layer?

04 November 2022 3,610 0 View

Is there any reaction factor or constant for liposome formation?

I want to learn what is the reaction factor or constant for liposome formation. We are going to simulate it by COMSOL, Diluted Species Model. But we need a factor for the addition of lipids. Maybe...

14 April 2022 3,960 3 View

Which parameters or tools can be alternative to PIP scoring?

Hi I'm working on two proteins, one of them carries a PTM. Except for haddock, many docking servers do not recognize PTM and do not process it. I have the same problem with PIP scoring when...

08 March 2022 6,622 1 View

How the electrical field affects liposome sand organic solvents?

Maybe I am asking the wrong questions, but I'm curious about how are liposomes, phospholipids, and organic solvents affected by the electrical field on microfluidic systems. What is the behavior...

02 January 2022 7,296 2 View

A glue for attaching polypropylene compound to metal?

I want to make a glue to adhere polypropylene to metal (for example Aluminium or stainless steel). This has to be partly air drying. Adhesive layer should be fairly thin. It can be applied as a...

27 September 2020 6,200 5 View

Can't the Python sklearn decision tree classifier correctly handle categorical attributes?

Hello, I'm trying to model my dataset with decision trees in Python. I have 15 categorical and 8 numerical attributes. Since I can't introduce the strings to the classifier, I applied one-hot...

24 July 2020 5,964 3 View

How can I apply position restraint to a bilayer during Umbrella sampling for PMF calculations?

Hi all, I have a couple of questions for PMF calculations in Gromacs. I placed a nanoparticle (NP) in the water region approximately 1nm away from the membrane and by using Gromacs pull code, I...

21 April 2020 4,033 4 View

How do I calculate bilayer thickness with Gromacs distance command?

Hello all, I carried out a molecular dynamics simulation for a coarse grained membrane system. I want to select the PO4 groups in top and bottom leaflets and calculate the vertical distance. How...

16 June 2019 9,542 4 View

any study on protective factors of care givers of dementia and resilience ?

need to review articles on care giving in dementia and challenges faced

12 August 2024 6,424 1 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

How do I replace a file with a more recent version of a paper that was uploaded to ResearchGate?

The paper in question is "Interpolation of Nitrogen Fertilizer Use in Canada from Fertilizer Use Surveys". This paper was very recently published by Agronomy (MDPI). Agronomy has, in the last day...

07 August 2024 9,934 3 View

E.coli contamination in human RNA seq data ?

Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...

06 August 2024 807 3 View

RNA later for the preservation of RNA in fecal samples at room temperature for one day (37°C)?

I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...

06 August 2024 1,367 3 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View

Frederic Lepretre

Hi Gül,

at the UCSC website (https://genome.ucsc.edu), you'll find some tools as the genome browser that contains enough optional tracks for your purpose (just choose the right species nd genome.

but in general, I advice you to take some times in this website and explore all possibilities to run genomics and genetics projects.

all the best

fred

Udhaya Kumar. S

Article An improved method for isolation of RNA from bone

Article A method for isolating high quality RNA from mouse cortical ...

Article Improved method for isolating high-quality RNA from mouse bo...

Check out these articles.

Thanks

Gül Otlu

Frederic Lepretre Thank you so much Fred! I am gonna give it a try. I checked the database and I need to take too much time on it cause i m not familiar with it. I have a gene list, now I am trying to identify their downstream genes to run RT-PCR, hopefully I can identify. Thank you for your suggestion again.

Udhaya Kumar. S Thank you ! My best labmate !