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My insert DNA (size 1.5kb) was gel purified sample with a good concentration (100ng/ul , 260/280-1.94 and 260/230- 1.90). I used pGEM-T easy vector (size-3kb) for ligation and followed...
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After isolation of genomic DNA from E.coli using phenol/chloroform method, the sample shows RNA contamination. (i) If the sample is treated with RNase enzyme is it necessary to remove the enzyme...
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