Hello everyone. I had human colon cancer samples preserved with RNA later at -80 ° C and I extracted the RNA using the ZYMO research KIT by removing the RNA later and proceeding with the extraction. Later I read the RNAs at the nanodrop and the concentrations were ok as well as the ratios (260/280 and 260/230 around 2). Later I analyzed the samples at the Bioanalyzer and I obtained very low RINs (around 2). Could anyone tell me how is this possible? What could be the problem? Thank you
Hello Mariarosaria
Nanodrop would not tell anything about the integrity of your RNA. It tells you only about two parameters: concentration and purity, that's all. Even the concentration might be biased due to presence of genomic DNA.
Regards
The reading from nanodrop only gives information about the nucleic acid present in your sample, which could be rna, not ruling out DNA contamination. The high value you got might be due to DNA contamination and not actual RNA. I will advise you perform gel electrophoresis on the RNA samples then you will be able to see if there is contamination by genomic DNA.
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