Resistance mechanisms- MRSA?

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How to grind your tissue for RNA extraction?

Hi fellows, I've started a project in a new lab where I have to extract RNA from mice liver, brain, and muscles. Upon dissection I immediately snap-freeze the tissue in liquid nitrogen and store...

19 February 2024 1,639 4 View

How do different infectious diseses impact fever resolution patterns?

Hi, I need an article/summary on different resolution patterns (including fall by crisis and fall by lysis). Thanks :)

07 February 2024 1,197 0 View

What is the best method for dissecting a frozen fish without thawing?

I need to extract the liver from frozen Emerald rockcod and sequence its RNA. To keep the RNA stable and prevent degradation, I would like to avoid thawing the fish for the dissection. However, I...

23 October 2023 6,131 2 View

What wet transfer conditions would be best suitable for a 5kDa protein (Aß42) in Western Blot onto a PVDF membrane?

Hi everyone, I've tried to transfer initially using a 20% methanol transfer buffer using 250mA max current for 70 mins, all I got was transfer of mid-high range proteins, I suppose all proteins...

25 July 2023 1,863 4 View

Is it okay to set my own ranges if the range is not mentioned in the scoring information in a standardized questionnaire?

Hlw I am doing a research on Homophobia among youth and parents for which I am thinking to use a standardized questionnaire- Homosexuality Attitude Scale (HAS), but the scoring information of the...

01 June 2023 4,366 13 View

¿La edad importa: Categorización de la fuerza laboral por generación y su impacto en el desempeño laboral?

Estimada red, estoy iniciando a desarrollar un articulo, me gustaria me brinden ideas para cosiderarlas en este nuevo proyecto.

19 March 2023 7,037 1 View

How can i assess the goodness of fit in GLMM with NB distribution?

Hello all, I've got a rather complex question concerning my regression. My data has the following properties. Dependent variable is count data and is overdispersed and consist of repeated...

22 February 2023 8,786 3 View

How many species of moulds are there?

I am trying to find an article on the diversity of mould species. They are found in the divisions Zygomycota and Ascomycota. Does anyone know how many genus or estimated number of mould species...

16 February 2023 9,059 2 View

Question about how to fix "No data on chk file." error for Gaussian?

Hello, I am running a dft calculation that keeps completing in less than minute giving me this error: "No data on chk file. Error termination via Lnk1e in /apps/gaussian/g16c01/g16/l301.exe" I...

06 January 2023 2,533 2 View

Can you scale down taqman qPCR gene expression assays from 20ul reactions to 10ul or 5ul reactions?

Usually taqman qPCR gene expression assays using taqman fast advanced mastermix require 20ul reaction volumes. I wanted to scale down reaction volumes to 10 or 5ul to save on reagents but I was...

21 June 2021 9,273 3 View

What is the best way to drop a plasmid?

Please address the best way to drop a plasmid. Background: I have a "bait" plasmid resistant to kanamycin and a "prey" plasmid resistant to carbenicillin. After many rounds of streaking on...

25 July 2024 2,532 3 View

My rosetta strain has chloramp resistance and i am transforming my plasmid with amp resistance but not getting colonies on chloramp/amp LB plates?

I have done the same procedure with BL21 and pLysE but i got colonies but multiple times on rosetta i did but not getting. can anyone explain what would have gone wrong?

12 July 2024 9,904 1 View

Quelles applications innovantes peuvent bénéficier des propriétés des géopolymère?

Les géopolymères offrent plusieurs propriétés intéressantes qui peuvent être exploitées dans diverses applications innovantes : 1. Construction durable : - Bétons à faible empreinte carbone -...

10 July 2024 7,457 1 View

What is alternative for PMMA?

If we want to introduce a material similar to polymethyl methacrylate "with the characteristics mentioned in the next paragraph" that can be added to a precursor and create a composite, which...

03 July 2024 2,371 1 View

How can we reduce the porosity of tungsten oxide (WO3) thin films during DC sputtering deposition (target: W, power: 100 W, 75 W, and 50 W)?

We are fabricating a tandem structure where a ~50 nm thick WO3 film is deposited between two metal films using DC sputtering. We use a mixture of Ar and O2 gases at different flow rates while...

01 July 2024 2,686 2 View

Hi i m workiking with hct116 resistant and naïve cells after passage half of my cells die can anybody help?

thanks

25 June 2024 4,667 0 View

Why carrier Concentration decreases while increasing Temperature ?

Hi All . Recently, I conducted Hall measurement experiments at high temperatures on highly p-doped diamond with a doping concentration of 2.5×10^20 cm−3 During these experiments, I observed two...

23 June 2024 4,763 0 View

Why carrier Concentration decreases while increasing Temperature ?

Hi All . Recently, I conducted Hall measurement experiments at high temperatures on highly p-doped diamond with a doping concentration of 2.5×10^20 cm−3 During these experiments, I observed two...

23 June 2024 6,583 2 View

Effect of post bake on PPR resist?

HI , i am concerned about any resist hardening effect of Post bake my LOR +PPR coated sample @90C for 5 mins. I wanted to do liftoff in acetone but the metals are hard to peel off. I cant use PG...

18 June 2024 5,741 0 View

Why should you convert a WGS to core genome? and what is a core genome?

I read a paper where they converted WGS to core genome to check for AMR through resfinder, any insights would be appreciated, new to bioinformatics.

10 June 2024 8,347 1 View