The plant is already known as anti-cancerous, but the biochemical study has not been done. However the active compound, from the plant has been researched already to find anti-cancerous activity in various in-silico studies, however no wet-lab is done. How should the road map of the studies can be carried out ?
you can proceed with the following procedure
1. by extracting pure active compound by different methods.
2. test on certain cancer cell lines. if results is good then,
3. Do some pre clinical studies ( mice etc.)
4. Finally clinical studies with humans
Regards
H.N. Nagendra Prasad sir, do i need to run a synthetic simulation of the active compound against the cancer protein even before proceeding to wet lab ?
In silico or simulation studies are very helpful, but they are just a step to clarify and facilitate the research.
Test the raw extract on cancer cell lines in vitro and chemically induced cancer in vivo, if the extract. If it showed anticancer effect, try to separate and purificate the effective ingredients and test them separately. Determine the LD50 of the effective compound in two animal species. Perform preclinical study. Then clinical study
thank you Ali Esmail Al-Snafi sir and Naïrouz Benzeggouta to claryfy my doubts, Thank you.
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