How can I keep fresh plant material for enzyme and phytohormone assays and gene extraction?

Greetings, I have to transport fresh vegetable matter for enzymes and phytohormones assay and gene extraction in a laboratory far from my laboratory, so how to keep this vegetable matter in its...

09 October 2019 10,044 5 View

What's the best method to inoculate seeds by PGPR?

I m doing a studies about the effects of PGPR on seeds and plants developing, by inoculation, so what's the best way to inoculates seeds by bacteria! Thank you

11 December 2018 3,525 0 View

What are the extraction and dosing techniques for bacterial osmoprotectants?

Greetings, I'm searching for methods to extract and dosing some of bacterial osmoprotectant such as ectoin and betaine glycine and proline from bacteria Thank you

11 December 2018 7,824 0 View

How find the same Osmotic pressure of two solutions of NaCl and PEG 6000 ?

I need to find the same osmotic effect (pressure) on a plant or seed exerted by two solutions one contained Nacl and other PEG 6000 ! how can i find the equivalence between NaCl and PEG 6000...

02 March 2018 7,345 7 View

How can i disinfect seeds without being imbibed or stressed by disinfection solution?

How can i disinfect seeds use to germination study under stress conditions (water stress or salinity) without being imbibed or stressed by disinfection solution? Thank you

02 March 2018 3,027 2 View

Does the disinfection of the soil make him lose these characteristics?

I need to work with disinfected soil, does putting this soil in an autoclave make it lose these characteristics? Is there any other techniques to obtain disinfected soil, without any microorganism?

31 December 2017 4,893 15 View

What's the best software to compare?

31 December 2015 5,650 0 View

Why don't we use colchicine to kill directly tumors instead of starving these cells?

11 December 2014 8,687 11 View

I'm looking for a protocol to stain and view chromosomes in metaphase?

I'm looking for a protocol for viewing - under a microscope - the chromosomes in metaphase stage, in a plant root after seed germination.

05 June 2014 2,039 8 View

Can we regenerate human organs?

Plants and some animals are characterized by the system of regeneration and repair of damage in their bodies. Is it possible to use this feature for regeneration of human organs and regenerated...

04 May 2014 4,324 10 View

What is the best way to keep an anaerobic chamber anoxic in the absence of hydrogen?

I'm a graduate student who works with an anaerobe, so I often work in an anaerobic chamber. I've been working on an assay that involves the steady-state of the quinone/ quinol pool. Anoxic...

03 March 2021 2,971 1 View

Good sites to recruit survey participants on a grad school student budget?

Any good recommendations for participant recruitment for online surveys on a grad school student budget?

02 March 2021 4,293 4 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Can NFL theorem be valid in infinite search space in ML?

NFL theorem is valid for algorithms training in fixed training set. However, the general characteristic of algorithms in expanded or open dataset has not been proved yet. Could you show your...

01 March 2021 1,189 3 View

How to define and get results (stresses) for certain points of a model in abaqus, using python? When the size of the model changes?

I want to do a parameter study of a aixsymmetric cylinder in Abaqus. I want to create several jobs where I get the stresses for 3 different points in my model. These points are defined. I wrote a...

01 March 2021 10,078 1 View

LOAD DISPLACEMENT CURVES Stiffness increasing in ANSYS non linear analysis. HOW CAN I CORRECT IT?

Hi. I am doing non linear FE analysis using a new material beam under flexural 2 point loading using ANSYS. I have defined a bilinear material model. The solution has converged. In results I am...

01 March 2021 9,911 3 View

Is there any research regarding how training and development is going to change in the era of Fourth Industrial Revolution?

As the organizations getting prepared for the fourth industrial revolution, to enhance the skills of their workforce what type of changes are necessary and going to occur in the training and...

01 March 2021 3,199 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Samples to study circular dichroism (chirality)?

I want to build a low-cost polarimeter to study circular dichroism (chirality). What type of samples can I use? I am looking for samples that can be found outside the laboratory.

01 March 2021 9,101 3 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View