What techniques do you use for hESC colony picking?

I'm growing hESC cells that are recovering from a transfection in a 6 well plate. They form colonies but I find it quite difficult to pick the colonies. When I detach them using a colony picking...

07 April 2018 1,553 2 View

What is the best approach for freezing and thawing HUVECs?

I froze some HUVECs in 10% DMSO and 90% FBS over a year ago. When defrosting I placed in a water bath until mostly thawed, then added warmed EGM-2 to cryotube before transferring to a 15 mL Falcon...

27 April 2015 7,081 6 View

Bethesda V -suspicious of papillary carcinoma

In a patient with multinodular goitre ( non toxic and no compressive symptoms) , with a single suspicious nodule (2 cm) on ultrasonography and cytology of that nodule as Bethesda V ( suspicious of...

01 January 1970 7,035 9 View

How to distinguish the host cellular receptors are functional in virus attachment or virus entry ?

If you find the plasma membrane protein can bind to virus attachment proteins, how do you know this protein in related to virus attachment stage or virus entry stage? As I know, we can perform...

01 January 1970 2,591 1 View

How to distinguish the host cellular receptors are functional in virus attachment or virus entry ?

If you find the plasma membrane protein can bind to virus attachment proteins, how do you know this protein in related to virus attachment stage or virus entry stage? As I know, we can perform...

01 January 1970 7,800 1 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Can we proceed to perform Two-way Mixed-ANOVA despite normality violations?

While testing assumptions, Shapiro-Wilk Test failed. However, I proceeded with the analysis and the interaction effect was significant. During our course- lectures, it was mentioned that Anova...

24 February 2021 228 3 View

How to run repeated measures in mvabund?

I have a dataset of fish species abundances collected from 6 different habitats over the course of 30 years. Fish were collected multiple times per year and environmental variables were recorded...

21 February 2021 3,838 1 View

ANCOVA controlling baseline in RCT - how to perform power analysis and sample size estimation?

Hi, I have a simple question. I am hoping to perform a power analysis/sample size estimation for an RCT. We will be controlling for baseline symptoms, and using post-treatment or change scores as...

19 February 2021 2,648 23 View

Am I doing the mixed ANOVA right wnen there is many missed values?

01 February 2021 4,695 7 View

The results of the repeated measures ANCOVA show no significant difference for within-subjects (SPORT types), can I now conduct a regular ANCOVA?

Usually a repeated measures ANCOVA is used for comparing results at different times. For my study, this is not the case as the within-subjects variable is a sports category (basketball or...

23 January 2021 2,605 3 View

Can I calculate the average from the data which are collected every 30 minutes for ANOVA?

I artifically elevated the air temperature in the heated plot by 3-celsius degree compared to that in the control for 7 days. I want to figure out the effect of the temperature manipulation on...

21 January 2021 1,185 8 View

Similarity between these two waveform? Similarity in terms of Shape?

Hi, These are the outputs generated by a system. Green-colored (nr) is desired output, while the system generates Red-colored (nf). I also have a dataset in CSV file. For this waveform, What...

21 January 2021 929 2 View

Repeated Measures Three-way-ANOVA Interpretation of Interaction?

Dear all, I am working on analysing a study Desgin consisting of 3 independent categorial variables and one continious dependent variable. All Subjects were tested for the same conditions. So I...

20 January 2021 330 3 View