I am working with tiger faecal samples for genotyping and one locus showing alleles for 110-134 across many individuals. I was just wondering whether they are the real one, despite a gap more than 20 bp between them! Thanks
Yes, those can be considered as alleles of that particular locus. However, to eliminate any doubt, you need to sequence the region that its been amplified by that pair of primers. All the best!
Is it a 2bp, 3bp or 4bp repeat? What is the distribution of allele sizes for the locus over the entire population (or for that locus in other populations)? It would not be that unusual for a very allelic rich locus to have an individual with two alleles at the extreme ends of the distribution.....e.g. if your population has alleles at that locus ranging in size from 110 to 134 for a 4bp repeat, then there are only 6 alleles, so individuals heterozygous at 110 and 134 is not a surprise. However, if you are not getting any other alleles at all in the population ranging between 110 and 134, then you might be having a problem with allelic dropout (but this situation could also arise through an extreme bottleneck event, which also wouldn't be a surprise for a tiger population).
Locus has 4bp repeat. I noted 5 alleles for this but with higher frequency at the ends. Thanks
- Badges
- Science topic
- Similar topics
- Biological Science
- Zoology