My Culture supernatant contains 1.25 % Tween 80 . I need to remove it for assaying my culture supernatant for various parameters. Any suggestions how can i do it ?
Hi,
Do you mean some stage of protein expression and purification?
I have no personal experience but It looks like its not so easy to remove tween 80. You can try the pierce columns after diluting your sample (they mention that tween 80 was not tested- www.piercenet.com/files/TR0019-Remove-detergent.pdf).
If its for purification, think it should also work if you dilute the detergent and concentrate proteins with centrifugal filters (egs. Low cut off centricon filters).
Good luck
Manoj
I need to do Hemolytic assay with the culture supernatant. Tween if present will naturally lyse the RBC so i need to remove it
Hi. I am now facing the same problem as you and I need to remove Tween 80 from my culture supernatant.
May I know how did you manage to remove last time?
Hi. The critical micellar concentration (CMC) of tween-80 is ~10 microM so you could use either dialysis or or filters that would retain the large tween-80 micelles while letting proteins go through.
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