Do amorphous materials have a higher tendency for agglomeration/coalescence/coarsening than their crystalline counterparts?

In the crystallization mechanism, particles often first go through an amorphous phase (where the units of the 'to-be' crystal are disorganized) before they reach their crystalline lattice...

25 October 2020 8,829 3 View

Permission to use the pictures from other journals in our review paper?

Dear all, Could you please share how we can get the permission from publishers like IEEE, PIER to use the pictures from their journals in our review paper? Thank you.

23 September 2020 8,355 3 View

Is there a general formula for calculating the polydispersity index for PSD data collected with laser diffraction?

With laser diffraction I have collected particle size distributions. Some samples are very monodisperse and some are very polydisperse. Is there one general index (like a polydispersity index)...

04 April 2020 5,331 3 View

What will be the effective permittivity and permeability of artificial magnetic conductor?

In case of metamaterial absorbers, the effective electromagnetic parameters such as permittivity and permeability should be equal. What will these values in case of high impedance or artificial...

19 March 2018 9,929 7 View

What is wrong with my PCR amplification?

I am trying to amplify the Mus musculus mitogen-activated protein kinase kinase 1 (Map2k1) gene for LIC cloning. Forward Primer: 5'-GACGACGACAAGATGCCCAAGAAGAAGCCGA-3' (Tm 57C) Reverse Primer:...

25 November 2014 2,588 14 View

Acrylamide does not polymerize

hello can any one help me with my SDS - PAGE polymerization problem. i am following molecular cloning by shambrook

29 October 2011 9,751 14 View

Can I arbitrarily take a cycle number of 40 for the gene that was not expressed at all in qPCR results calculation?

I ran RT-qPCR for a control and a treated group and it appears that my gene of interest was not expressed at all in any of the subjects in the control group. So I was wondering if I could...

20 February 2021 6,243 10 View

Does anyone know if the Quantstudio 3 qPCR machine requires a passive reference when Using SYBR? I ran a qPCR and got the plot in the attached file.?

I know that my target is in my sample as I extracted it from cell culture myself. These plots show some amplification. The first one seems O.K for the first 36 cycles but then collapses at the...

14 February 2021 2,809 2 View

Are there any exception like work hardening in metals elastic limit due to dynamic loading?

The samples made out of spring steel are taken from same lot. The specimens were shaped following heating to forging temperature - Forming- Hardening -Tempering methods. After storage of almost 6...

11 February 2021 952 6 View

Having trouble with qPCR amplification plots. My curve's never seem to reach a plateau. Any ideas?

The parameters of the curve all seem acceptable, typically reactions results are above 0.9852, -3.1 to -3.6 slope, and 90-110% reaction efficiency. The results seem to be what I would expect from...

04 February 2021 1,138 3 View

Queries regarding the rain flow counting algorithm?

02 February 2021 9,790 2 View

PCR primers bind to specific DNA at specific temperatures but not other temperatures?

I ran PCR in order to analyze a P-element excision on a mutant drosophila line. My results were not conclusive, however that is beside the point. I ran a temperature gradient in order to optimize...

25 January 2021 9,164 11 View

HPLC: how to calculate violaxanthin area if it overalps with neoxanthin?

Does anybody know of, or have a method suitable for HPLC analysis that allows to separate violaxanthin from neoxanthin? I want to quantify the xanthophyll cycle (violaxanthin, antheraxanthin and...

21 January 2021 5,775 5 View

the product size is 191bp would it be a problem during conventional pcr?

I have designed a primer for Bacillus subtilis sfp gene. As I have mentioned the size is 191 bp. however I need some suggestions regarding PCR temperatures and cycle length. As the it is smaller...

09 January 2021 8,309 4 View

In a random graph, the number of paths that cannot be extended in an hamiltonian path, can be roughly be separated in two equal parts ?

In a random graph, the number of paths that cannot be further extended in an hamiltonian path or an hamiltonian cycle, can be roughly be separated in two equal parts? I have an idea for solve for...

07 January 2021 9,782 2 View

Does cleaning up cDNA after reverse transcription reduce late cycle background?

I've been wondering if removing the random primers from the reverse transcription reaction either with a column or precipitation before using it as a qPCR template reduces the background a bit in...

25 December 2020 6,258 4 View