Dear Anmol Gupta

The following text is included in the attached article:

• For ACC deaminase activity isolated strain was grown in tryptic soya broth up to late log phase at 30 °C in an orbital shaker at 150 rpm for 24h. After centrifugation cell pellet was washed with 0.1M Tris-HCl (pH 7.6) and finally suspended in DF minimal salt medium with 3 mM ACC as sole nitrogen source. The enzyme activity in cell free extract was determined by measuring the amount of -ketobutyrate generated by enzymatic hydrolysis of ACC (Saleh and Glick, 2001). The protein concentration of suspension was determined by Lowry method (Lowry, 1951). The standard curve of -ketobutyrate was determined at 540 nm to standard curve of -ketobutyrate ranging between 0.1 to 1.0 µmol.

Thanku very much to all and please let me know regarding calculations for ACC deaminase activity of bacterial isolates. Good morning have a nice day