Regarding calculation of chlorophyll and carotenoids content.

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Vimal Kumar Semwal

I Used acetone method and think is better

Abeer I. Shabana

There is no fixed rules, as several factors control the efficiency of pigments extraction e.g., plant species, extractant solve and extraction duration. I used methanol and acetone and observed that methanol was more effective in extracting chlorophyll from many vegetable leaves.

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Gagandeep Kaur

For Chlorophyll and carotenoids, acetone method is better according to me. I opted this method and results are promising.