Reduction of the production flow due to the deposition of carbonate scales in the tubing?

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TdTomato detection using RNAscope?

Has anyone tried to use RNAscope to detect tdTomato in FosTRAP::tdTomato mice? I wonder if there's an optimal timing on which the tomato expression might be detected after tamoxifen injection?

12 December 2022 4,925 0 View

Has anyone ever managed to get males from the C. elegans strain hsp-4::GFP (SJ4005)?

I'm having trouble getting males from the C. elegans strain hsp-4::GFP (SJ4005). I tried HS submitting L4 animals to different temperatures and times and also him-14 RNAi and neither approach...

28 July 2022 7,728 7 View

RAW 264.7 cells detaching from the plate and appearing not to polarize?

I am seeding 1 x 10(5) or 2 x 10(5) in a 24-well plate with DMEM 10% FBS for two days. Polarizing cells with M0, M1 (1ug/ml LPS + 25 U/ml IFNy) and M2 (40ng/ml IL-4) for 24 hours. I have groups...

12 October 2021 2,403 1 View

ABO allele database ?

Hi I am looking for a recent human ABO allele database. I know the BGMUT (NCBI) database was discontinued years ago and don't really understand why. The 1000 Genomes Erythrogene database seems...

28 January 2021 7,639 3 View

How to build a protein complex?

I want to bind alpha-Hemolysin to ClpP using specific linker molecules. I have the .pdb files of each of these individually, but I'm having some trouble assembling the system. I want to bind the...

19 May 2020 7,438 0 View

Anyone here has used an Uv/vis spectrophotometer named Nabi by Microdigital?

Hi! It is very similar to Nanodrop. It worked very well, however, the display doesn't turn on today. Do you know this equipament? Anyone can help me, please? Thanks!

20 November 2019 4,032 3 View

How to print AOMDV route list?

Dear, I needed to print the list of paths the source received in AOMDV. I also need to change the way the route is selected. Can someone help me?

04 November 2019 2,578 0 View

How add new field in the routing table on AOMDV?

Hello. I need to add a new field within the Aomdv routing table. For this, I create the field within the AOMDV_RTABLE.H and arrow it in AOMDV_RTABLE.cc, is it correct.? My question is how I can...

12 August 2019 1,300 0 View

Can with High Glicemic Rates Induced by Stress be Confused with Type 1 Mellitus Diabetes?

1 - Can continous stress induce high glicemic rates in humans and trigger diabetes type1 ou 2? How those possible stress induced problens are related to age? Can those high glicemic rates or...

29 May 2019 2,475 3 View

Exchanging detergents using dialysis, is it possible?

I need to exchange detergents by dialysis, from CHAPS (18 CMC) to Tween 20 (2 CMC), my protein has 2 MDa. 1) Would it be possible to do this by dialysis? 2) Suggestions for the conditions of...

15 March 2018 2,734 2 View

Is it possible for the soil pH to be around 8, but with negligible amounts of carbonates, bicarbonates and exchangeable sodium?

Can this pH of 8 be due to the presence of other substances? Or do I have an error somewhere in the analysis? Thank you!

26 June 2024 9,093 4 View

Why neutralization is necessary in the method for the determination of methylglyoxal?

In most colorimetric determinations of methylglyoxal, there is neutralization using saturated potassium carbonate, and perchloric acid is added to the final reaction. I would like to understand...

23 June 2024 2,074 0 View

Accumulation of water inside the IRMS after carbonate analysis?

Hey guys! My name is Lígia and I work with GasBench II/IRMS (Delta V Advantage). We have a problem with our instrument. The water recorded by m/z 18 is around 20V. We checked the Nafions and they...

09 June 2024 1,065 4 View

What to prepare of 0.05 M bicarbonate carbonate, ph 9.6?

I want to prepare 0.05 M bicarbonate carbonate, pH 9.6 as coating buffer for ELISA. Could anyone help me with right recipe, procedure, and or reference in the preparation? Thank you in advance.

10 May 2024 4,906 4 View

Why does the thermal decomposition beavior (TGA temperature) of propylene carbonate is much lower than its boiling point?

We are recently studying a materials containing propylene carbonate (PC) as co-solvent. And the TGA results show it started to decompose below 200 C (~110 -150 C). When we analyzed each components...

02 April 2024 5,942 0 View

How to prepare 1M NaClO4 in propylene carbonate?

I want to prepare NaClO4 electrolyte for Na ion batteries, What are the necessary calculations to consider while preparing the electrolyte batch?

25 March 2024 3,029 2 View

Could you help me to identifying this benthonic foraminiferal species?

this thin section sample which taken from Late Campanian - Maastrichtian age carbonate Formation Northern Iraq.

20 February 2024 9,023 1 View

Hi , could you help me in identifying this benthic Foraminiferal species ?

this thin section which taken from Late Campanian - Maastrichtian Carbonate Formation Northern Iraq.

20 February 2024 6,855 2 View

How to analyze the chemicals and gases of root decomposition within sandy soil of desert?

Some kinds of desert plants roots are decaying in desert sandy soil to produce/cause carbonate precipitation around the roots. Do you have any ideas about measuring the released gases and special...

19 February 2024 3,917 0 View

Identification of millimetered structures in sediment from core (Uzbekistan lake) ?

We found two strange structures in fresh watered lake from elevation higher than 1500 m a.s.l in Nuratau and Zarafshan ranges: 1: cristal in X or in "star-shape". Any ideas ? 2: fluter cylinder...

18 February 2024 1,306 2 View