I found that alkaloids normally appeared as green spot on tlc plate under long wave UV. Then how about red spot? I'm doing isolation of constituents from plant materials, is the red spot needed to isolate out normally? Does it posses phytochemical properties? Cause I'm thinking is it pigments like carotene or chlorophyll.
I think the red spots could be chlorophyll and related structures. If you have LC MS MS, I think it will be nice to analyze your extracts using it. Best regards
Ok thank you! I did LCMS MS of the crude extract before this, will check on it later.
Red spot means it is different constituent than your desired component with green color. TLC can only tell you this that it is different. You will need to have advanced chromatographic analysis for further determination like HPLC or LC-MS.
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