Suggestion: use Lowicryl resin for embedding; carry out post-staining of sections with ruthenium red. I would expect high contrast. For details, ask Wolfgang Muss, Salzburg/Austria (also member of ResearchGate). We had discussions on this approach just a few days ago (s. Questions and Answers). He has also access to relevant literature.

Kind regards,

Frank Mayer

You can try to add 0.3% ruthenium red to standart fixation. See Arvid B. Maunsbach, Björn A. Afzelius

Biomedical Electron Microscopy: Illustrated Methods and Interpretations p. 44, it is available on Goggle.

Thank you for your suggestions

M.R. (Pete) Hayden, Md

Maybe this is helpful

Article Assessment and Imaging of the Cerebrovascular Glycocalyx

Request for PDF copy

Thank you Roel, Alexandra, Frank, Mohamed

All of your recommendations will be taken into consideration and I will

first use a Alcian Blue

the Alcian Blue8GX alone in immersion-fixation and non-perfused.

Alcian Blue 8GX +

2.  Lanthanum that has been used and/or lanthanum plain without red.  

in combination or maybe even +

3.  ruthenium red

Also some have suggesteed

specifically 

4. Lowicryl resin  instead of standard  acrylic resin -

it all will be trial and error..

YOUR THOUGHTs and recommendation have all been greatly appreciated.

M.R. (pete) Hayden