Hello Everyone,
I"m looking into independently learning PEAKS and Perseus on my free time and wondering if anyone can recommend a tutorial or demo?
How many nucleotides can be placed between the overlap and annealing section of the final reverse primer for NEB assembly (Gibson assembly)? P/T2A ok? This would be the last reverse primer that...
10 November 2017 7,333 4 View
In the past, I have obtained varying results with Phusion, but have been told there are other polymerases that can deal with the GC richness of some regions in genomic DNA. Do I really need to buy...
07 August 2016 3,675 9 View
Initially, our paper was reviewed by three people, and then a 4th. 3 suggested publication with 2 suggesting only minor edits; one was strongly opposed. After the rebuttal, the same three clearly...
06 July 2016 2,313 9 View
I would like to stably express these genes in cancer cells while removing expression of wildtype protein.
10 November 2013 4,647 4 View
The plasmid is siRNA resistant, and I'd like to show a rescue in cell cycle phenotype, so it is important to have plasmid expressed first or very soon after si tfx.
10 November 2013 3,077 11 View
“Here is a thought experiment. Let's place Rodolpho Llinas's jarred-brain on top of a body (Fig. 1). I bet Llinas would argue that his jarred-brain retains its own consciousness, and the android...
11 August 2024 2,483 1 View
"PUBLISHING IN A SCOPUS JOURNAL" Researchers are now at a cross road. The critical need to publish in a Scopus or ISI, etc journal is ever vital. Journal Publication fees must be submitted....
10 August 2024 8,621 1 View
Hello experts, Does anyone know any free software about retention index prediction ?
08 August 2024 7,403 2 View
The rate of glucose consumption by the neocortex is reduced by over 80% during anesthesia (Sibson et al. 1998), which disables the synapses (Richards 2002) that are inundated by glial tissue (Engl...
08 August 2024 3,118 0 View
Larger brains, which typically contain more neurons, store and transfer more information (Tehovnik and Chen 2015), but the precise relationship between number of neurons and information has yet to...
05 August 2024 1,238 2 View
I need the python code to forecast what crop production will be in the next decade considering climate and crop production variables as seen in the attached.csv file.
05 August 2024 2,977 3 View
Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...
05 August 2024 6,247 3 View
I have an antibody binding generic protein and I need to compare its activity in a free and immobolized form. I understand that there are a number of methods to determine Kd value of a free...
05 August 2024 5,311 0 View
Hello everyone, I am currently working on a research project that aims to integrate machine learning techniques into an open source SIEM tool to automate the creation of security use cases from...
04 August 2024 3,196 2 View
When the entire neocortex is ablated in rodents, although they are still able to swim, all the limbs move continuously and asynchronously (Vanderwolf 2006; Vanderwolf et al. 1978). Normal animals...
03 August 2024 835 3 View