Looking for recommendations on what to use for imaging spheroids and organoids using a confocal scope. Would it be feasible to carefully transfer spheroids to glass bottom dish with coverslip, or better to use glass bottom microplate? Or another method altogether? I am experienced in the formation of spheroids but not so much the characterization. Thanks in advance
The easiest is to culture them in these (or similar) 8 well slide plates:
https://ibidi.com/chambered-coverslips/13--slide-8-well-ibitreat.html
You can place a 20-25 uL Matrigel dome and perform fixation and immunofluorescence directly in plate and image with an inverted confocal
Hi Karl, sorry to barge into the discussion, can I ask you which clearing technique have you used? I have spheroids with brain cell types that are almost 2 mm in diameter. I realize there are several kits available and I need to check which one suits for my purpose. I plan to do 4 color fluorescent staining on these spheroids then image using regular confocal microscope. Thanks!
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