Why the nucleic acid gel melted after running for 30min?

Hi all, I proceed with nucleic acid gel electrophoresis recently several times, but a wried phenomenon is they always melted after running over 30min. My gel concentration is 1% percentage...

22 February 2021 9,896 3 View

How to calculate trapping parameter of the deconvolution peaks?

28 July 2020 2,413 2 View

Have any professors ever done research about the expression of fusion proteins in Drosophila S2 cells?

1. How to scale up the expression of fusion proteins? 2. How to improve the purity of fusion protein to 90%? I have tried affinity chromatography, but the purity by SDS-PAGE was not satisfied....

04 November 2019 8,479 6 View

How to calculate the active area of CNTs modified glassy carbon electrode?

"The active area of so porous electrode as the authors used could not be correctly evaluated using the Randles-SEVCIK formula. Just because the depletion layers of active places may overlap." How...

28 October 2019 1,782 3 View

Future of bioinformatic analysis in plant science ?

please share your idea regarding the future of bioinformatic analysis in plant science? recommend the preferred PC and MAC programs for the beginner ,and for the advanced users? as well as any...

24 October 2019 8,814 4 View

Bad performance of RQ-PCR standard curve constructed by plasmid

Hi guys! Recently I encountered a problem about the Albumin (ALB) standard curve which was used for normalizing my samples. The ALB standard curve was constructed by serial dilutions of plasmid...

25 July 2017 8,762 3 View

Where to find the definitions of thin plate, medium thick plate and thick plate?

10 November 2015 3,459 0 View

How can I get the free GIS data of China?

16 May 2015 3,254 2 View

How to prepare the solution for ternary CdSexTe(1-x) thin films??

Can anyone explain in brief how to prepare the solution for ternary CdSexTe(1-x) thin films e.g. CdSe(0.6)Te(0.4) thin film?

06 October 2014 4,718 2 View

Can anyone suggest a reference electrode for electrodeposition?

Can anyone suggest a reference electrode for electrodeposition in aqueous solution? Which one is better between a Saturated Calomel Electrode (SCE) and an Ag/AgCl reference electrode?What is the...

28 April 2014 1,889 21 View

Ideas for Phd's Thesis on pricing (Marketing)?

Hi everyone, I'm studying Marketing and I would like to write my PhD thesis on the topic of pricing. Any specific ideas?

02 March 2021 9,706 5 View

12% ledeburitic cold work tool steel, etched for revealing retained austenite, any help for clarifying?

Hello everyone. Given microstructure image belongs to a 12% ledeburitic cold work tool steel (annealed and tempered). The sample was etched with picral (100 mL ethanol and 4 g picric acid). I...

02 March 2021 4,119 3 View

Which is the most efficient etching solution for nickel based alloys?

Which solution reveals the microstructure and features of nickel-based alloys?

02 March 2021 2,221 3 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration forms?

Warm greetings! My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology focusing on Human papillomavirus). At this stage, I have almost completed a clinical sample (Cervical swabs)...

25 February 2021 3,612 3 View

What kind of steel moulds are used for hot pressing metal powders?

My main concern is that the metal powders should not weld to the mold surface and I should easily be able to eject out the pressed samples. I am looking for alternative for graphite moulds. The...

22 February 2021 2,965 4 View

What does it require to be ready for e-Commerce in developing countries?

Most developing countries are not ready for e-Commerce? What dimensions and indicators are there to measure e-commerce readiness of a nation? Please share framework or model and evaluation...

22 February 2021 8,132 6 View

What are the LAB methodologies (procedures, equipment etc) for CO2 enrichment experiments to study crop responses?

I am doing a proposal for a research entitled 'GRAIN YIELD AND QUALITY RESPONSES OF WHEAT GENOTYPES UNDER CO2 ENRICHMENT AND EXTREME EVENTS' I will be using climate chamber and I would like to...

18 February 2021 6,494 4 View

Reliable and Valid Instrument for Causes of Medical Errors?

Is there any questionnaire/survey that measures the causes of medical errors? Example: physician negligence vs. systemic issues. Looking for a reliable and validated instrument. Thank you!

15 February 2021 2,874 4 View

How do I categories into groups on SPSS?

I am using the 'Revised Cyber Bullying Inventory'. This instrument has 2 sub-scales (bully scale and victim scale). From the responses, they will be divided into 4 groups (pure cyberbullies, pure...

13 February 2021 5,787 4 View