I'm looking for procedures to rear entopathogenic fungi in laboratory conditions (Metarhizium anisilopa, Beauveria bassiana, Paecilomyces). Can they all be grown on the same medium or are there different possibilities? Are there procedures which give all the information about medium, temperatures, growth temp, the best way of collecting conidia?
It depends on what you want to do. Do you want to make observations on the vegetative growth? Or maybe you want a good sporulation? I could help you if you'll give me more details. You'll find a lot of papers on this subject. But first you have to know what you want to do with these strains.
Anyway, all of them works well on PDA, but ...works very well on other media too:)
Hello Mihaela, thanl's for a responce. It's very funny, because I work with Gerben Messelink and I just heard from him that you come to work with us in April. I think the best will be to talk than. I am going to test some entopatogens on mealy bugs. I hope you can help me. See you in April. Greetings, Ada
yes, indeed, Ada, I'll be there in April:) world is such a small town!!!! I'll help you with all I know!! We could work together, because I'll test some entomopathogens too. Cant wait!! Please tell me anything you need to know. My e-mail is [email protected]
See my papers in the web on entomopathogenic fungi, using my full name: Alex Enrique Bustillo Pardey
Dear Ada
The fungi you mentioned in your question are not fastidious and they can be grown on ordinary media such as PDA and SDAY. For prolonged rearing they can be cultured on weak media such as PCA under cold environment (under 10 degree centigrade)
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