in this link:

https://www.thermofisher.com/co/en/home/life-science/pcr/real-time-pcr/real-time-pcr-assays/snp-genotyping-taqman-assays.html

tipiyng of rs1051730., after click en search.

after click in  assay ID. and this is your information:

Context Sequence [VIC/FAM]:

AGCAGTTGTACTTGATGTCGTGTTT[A/G]TAGCCTGGGGCTTTGATGATGGCCC.

VIC is allele A and FAM is allele G.

Best regards

Leonardo

 Using the channel that detects the fluorescence of each fluorochrome (VIC or FAM), then you must know which base of the SNP is marked with VIC and which with FAM.

Thank you, Leonardo beltrán

Ok, but how we know which base of the SNP is marked with VIC and which with FAM?

Abdolhaim,

Did you use taqman tests pre-designed by applied biosystems or thermo? or Were probes designed and synthesized by demand?

Leonardo beltrán

We used taqman tests pre-designed by applied biosystems, for CHRNA3 gen and rs number is rs1051730.

Thank you.

in this link:

https://www.thermofisher.com/co/en/home/life-science/pcr/real-time-pcr/real-time-pcr-assays/snp-genotyping-taqman-assays.html

tipiyng of rs1051730., after click en search.

after click in  assay ID. and this is your information:

Context Sequence [VIC/FAM]:

AGCAGTTGTACTTGATGTCGTGTTT[A/G]TAGCCTGGGGCTTTGATGATGGCCC.

VIC is allele A and FAM is allele G.

Best regards

Leonardo

Dear Abdolhalim,

In order to keep all this information together you should introduce all this data into the software (I use QuantStudioRealTime PCR software, the software for 7500 is similar) to create your library, as well as descriptive information regarding the assay you use. It will be very useful once you will increase the number of SNPs to check (we work on almost 20 SNPs for about 4000 samples)

Let me know if you would like to do this, I can prepare some printscreens for you and post them on Research Gate.

Good luck!

Dear Levitchi,

Thanks for the guidance. 

if possible please, send some print-screens in research gate.

Best regard.

Dear all

Thank you there.