Good morning, I urgently need to perform ddPCR analysis on some samples using a QX200 Droplet Reader that's been sitting in my lab for a while. A technician came in last month to set it up and I successfully performed an experiment last Friday. On Monday, though, I switched on the Reader and noticed that none of the PCR plates I had prepared where recognized by the instrument. The green light that usually flashes green when you insert a plate doesn't light up and the software doesn't allow me to start the run. I used original Bio-Rad plates that were successfully recognized when placed in another QX200 droplet reader from a different facility. As suggested by the technician, I unmounted and cleaned the plate support, but nothing changed. We replaced both the waste and oil bottles, but without success.

Perhaps some of you fellow researchers have experienced a similar issue with this device? Thank you so much for your kind advice.