Analysis steps:

1.Sample is crushed.

2.Supercritical fluid extraction use CO2 and 95% Ethanol.

3. Add known quantity caffeine after if sample is not contain caffeine.

4.Run GC/MS for determination of the flavonoid type and quantity.

Rutin and quercetin are the same, yes you may use rutin instead

Rutin and quercetin are NOT the same.

Yes you can use quercetin dihydrate in place of quercetin as standard, just keep in mind the difference in molecular weight.

Chemically there is a difference between the two compounds but both (rutin and quercetin) are belong to flavonoids classe in this case you can use all of them in the quantification of flavonoid contents.

You can use both substrates equally. The error you make due to the different molecular weight, is more or less negligible, when estimating the total flavonoid content of a plant extract....

Analysis steps:

1.Sample is crushed.

2.Supercritical fluid extraction use CO2 and 95% Ethanol.

3. Add known quantity caffeine after if sample is not contain caffeine.

4.Run GC/MS for determination of the flavonoid type and quantity.

Dear All, where are rutin, caffeine or whatever else mentioned in the original question?

Nidhi asked a very simple question. She has quercetin dihydrate but not pure quercetin. For some reason Aly R Abdel-Moemin recommended to use rutin as a standard and all discussion went in a wrong direction related to rutin.

Dear Nidhi, the answer to your question is YES! That's all.

Yes you can use it but take care molecular weight.