11 December 2020 3 9K Report

Du type NVivo, etc? Y-a t-il des programmes français performants?

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How to deal with Endogeneity in Discrete Choice Models?

Hello, i need to estimate a control function in order to deal with price endogeneity that appears in the attributes of the alternatives. Finding the auxiliary variable (derived from the control...

01 March 2024 5,048 0 View

Calculation essential agreement ?

For the calculation of the essential agreement, how do you consider values as ">8" or "

27 July 2023 7,428 0 View

Dear all, I was wondering if anyone knows whether MGLtools and Autodock can run on Mac OS?

I have tried to download the 64-bit version of MGLtools however, as many times as I have downloaded and uninstall the programs, an error arises that the app needs to be updated. Thank you.

03 March 2021 6,799 2 View

What cartoon-making software can you recommend (CC licenses)?

Practical recommendations based on your personal experience (required skills, variety of pre-programmed templates, etc) would be the best. Field of interest: education, business, and interior...

26 April 2020 4,921 2 View

Multiple linear regression after Principal Component Analysis?

I am currently running a statistical on a complicated set of data and after completing a PCA and deriving with a number of factors (18), I would like to run a multiple regression analysis with...

14 January 2020 7,220 4 View

How to measure temperature stress to invertebrates and rates of their predatory mortality in artificial bivalve beds?

Hello everyone ! I am designing multi-factor experiments that manipulate the environment in the absence and presence of ecosystem engineers along a environmental stress gradient. I would like...

26 May 2019 1,569 3 View

R vs Python for Discrete Choice Analysis?

What is your opinion, about data analysis using R or Python? I am going to analyze data using Discrete Choice Analysis. I know that both languages offer this potential but I would like to hear...

26 February 2019 5,674 4 View

How w do you think non cash rewards effects the attraction, retention and motivation to the Tech work force?

Am doing my dissertation on the Tech work force in the igaming industry and would like your intake on this. Thank you

10 October 2017 4,971 1 View

Are macrophages RAW 264.7 able to produce TGF-B?

I would like to know what cytokines are produced by macrophage cell line RAW 264.7. And if at baseline (no stim) RAW 264.7 is capable to produce TGF-B1! I appreciate if anybody could help me with...

10 March 2017 4,071 2 View

Storage frozen sections of cryosectioned paraformaldehyde-fixed tissue at 4 degres?

Usually i'm reading that cryosections should be stored at -80 or -20? Although if tissue was fixed with paraformaldehyde before processing for cryosection, the slides might be stored at 4 degres....

21 June 2016 5,194 4 View

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The Optimal Experimental Approach for Gene Function Analysis?

What is the most suitable experimental setting to understand the consequences of a particular gene: (1) targeted degradation of the specific transcription factor (TF) of the gene, followed by...

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When using NVivo software for data analysis, is it necessary for the researcher to also manually analyze the data?

it's my first time using NVivo software.

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How to we see transcription activity of the bhlh79 family is at the N-terminal or C-terminal?

I'm a PhD Student. From Northwest Agriculture and Forestry University China. My Problem is this I start my work on bhlh79 transcription factor gene. I do my Y2H 5 time but the colonies appear on...

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What happens in snapTotal- seq?

What happens in snapTotal- seq? (Single-cell Total-RNA Profiling Unveils Regulatory Hubs of Transcription Factors https://www.nature.com/articles/s41467-024-50291-3) In this protocol, several RT...

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Is it possible to generate a (semi-)permanent "transcription bubble" within a replicable plasmid?

I was wondering if it is possible to form a permanent open "ssDNA bubble" similar to a transcription bubble (>13 nucleotides) within E. coli. These criteria are important: 1. Open ssDNA...

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Is DNase I (RNase-Free) necessary to be used before cDNA synthesis?

I want to isolate RNA from bacillus subtilis using the QuickRNA Fungal Bacterial Miniprep Kit from Zymo Research and further reverse transcriptase to cDNA using the ReverTra Ace qPCR RT Master Mix...

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Can you publish your dissertation after the data has been destroyed?

Hello, Can I publish my dissertation even though the original data has been destroyed in line with my university's ethical procedure? Data was to be destroyed upon completion of my degree. I'd...

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How many interview participants should be quoted/mentioned in analysis in a mixed-method design?

Hi Everyone, I'm conducting research on culture shock using a mixed method design (Questionnaire + Interview). I have 60 Participants in Questionnaire and 10 participants (out of 60) in the...

13 June 2024 5,190 1 View

Why my in vitro transcription doesn't work?

Hi everyone! I am currently doing In vitro transcription of two different RNAs, one is ~90bp size and another one ~200bp. I am using 2μg of DNA template, the reaction is kept for 3 hours at 37...

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Can you help me with some problems I am having with dsRNA production?

The desired gene region was amplified by PCR and the resulting PCR products were cleaned. Bands were observed in an agarose gel and measured in a spectrophotometer. Then, transcription was...

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