Can anyone help me how to determine the level of attachment of amino acids onto Wang Resin (loading capacity 1.2 mmol/g) by quantifying the Fmoc release in SPPS?
Dear Papori It should help http://www3.appliedbiosystems.com/cms/groups/psm_marketing/documents/generaldocuments/cms_040640.pdf
best
Dear Papori,
You may use 300nm wavelength for absorbence and 7800 cm 472 −1 M−1
dm3 extinction coefficient for calculation of fmoc release
you may see this article for your reference http://www.ncbi.nlm.nih.gov/pubmed/25548942
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