Can anyone help me how to determine the level of attachment of amino acids onto Wang Resin (loading capacity 1.2 mmol/g) by quantifying the Fmoc release in SPPS?
Dear Papori It should help http://www3.appliedbiosystems.com/cms/groups/psm_marketing/documents/generaldocuments/cms_040640.pdf
best
Dear Papori,
You may use 300nm wavelength for absorbence and 7800 cm 472 −1 M−1
dm3 extinction coefficient for calculation of fmoc release
you may see this article for your reference http://www.ncbi.nlm.nih.gov/pubmed/25548942
I am working with a N20 mer (20 mer random region) aptamer library with a total length of 56 bps. I am facing the problem of higher sized ds DNA PCR products forming in my SELEX cycles. The size...
04 May 2018 856 3 View
I am using MicroCal ITC 200 (GE) and performing an interaction study between a DNA aptamer (12 kDa) and a peptide ligand (1.91 kDa). How can I explain an ITC curve with both negative and positive...
11 December 2017 9,100 5 View
How to calculate the RMSD values for a MD simulation using MOE?
07 August 2021 0 0 View
When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.
16 June 2021 0 0 View
Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...
03 March 2021 5,978 5 View
I'm involved in a study of odor control technologies for municipal wastewater treatment plant. One of the control options involves a chemical 2-stage (acid/alkaline) packed bed scrubber. The...
03 March 2021 3,661 2 View
I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...
02 March 2021 4,443 2 View
Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...
02 March 2021 4,569 4 View