My colleague wants to purify CD8+ T cells to do Elispot. I speculate that it could be difficult because it needs APC presence in the Elispot Culture. Could somebody provide comments if it is reasonable to use purified CD8+ T cells for Elispot? Thanks
You can definitely use purified CD8+T cells for Elispot. You are right T cells do need APC for antigen presentation, but if you recall, all nucleated cells have MHC-1, thus CD8 T cells can present antigen to other CD8 T cells, although the response would be weaker when compared to professional APCs, but you should definitely find the spot in Elispot.
Hi,
like Rakesh Bakshi said, you should use APCs. I most say, that I do not really understand your question, because for a typically ELISPOT you need APCs and the Antigen to get the recall of the T cells... In this way were are getting a lot of spots from purified CD8 cells after TB infections..
Dear Rakesh and Jochen,
Thank you for your comments. Why I just see your answer now? Anyway, your answer is still important learning for me.
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