I use the protocol of Kubori. T 1997 for B.subtillus (the protocol is attached). I don't manage to get any bands following the CsCl ultracentrifugation (there are only brown pellet on the bottom). The spheroplasts lysis (upon addition of Triton) in my samples takes 2-5 days until the suspension clearance. Should I buy CsCl for ultracentrifugation (I use CsCl 99.9%)? What could be my problem?