what is the protocol to conduct a pathogenicity test for soil-borne pathogens like sclerotium rolfsii
When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...
28 February 2021 2,133 3 View
We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...
28 February 2021 1,149 1 View
Hi, I am doing a review and hopefully meta-analysis for my Master thesis but I have the following problems: - Some studies only give the difference between end trial and baseline data and other...
22 February 2021 1,115 4 View
In my opinion, the RG site is useful in solving scientific problems and shows the researcher's ability. Besides, the separation of the H-index from the self-citations and the overall index is an...
22 February 2021 3,894 6 View
Hi, i am conducting a Monte Carlo simulation for my new distribution but the MSE values are increasing while the sample size increase, does any experience this issue before and what should i do...
22 February 2021 3,680 4 View
I have a basic knowledge on Python, R and MATLAB in analysing data. Among those, which is the best and user friendly? Which one should I learn deeply to analyse data?N:B: I want to learn data...
20 February 2021 3,950 15 View
the samples are not completely soluble - just swelled the solvents that's mean the sample may be partially soluble any suggestion, please
16 February 2021 8,801 3 View
We collected faecal samples from various species that we want to analyse their microbial communities' DNA via Next-Generation Sequencing. The problem is that due to budgetary constraints, we will...
16 February 2021 5,466 4 View
can any one suggest me other than classification, how deep learning technique can be used in plant disease detection in rice plant? could a research work continued from fungal,bacterial...
07 February 2021 470 2 View
When you use RNAseq I understand that it must follow certain conditions when you use it. One of them is the insertion of a polyA tail. The insertion of the polyA must include then...
05 February 2021 3,770 4 View
severity index, measuring method and procedure detailed
01 February 2021 1,160 11 View
I want to compare the RBD of all pathogenic viruses? Is there a database or manuscript that anyone knows of where I can access these?
25 January 2021 3,843 4 View
Hello, Most hand sanitizers contain 60-80% alcohol. Alcohol destroys organisms including bacteria, fungi, protists, and enveloped viruses by breaking down the plasma membrane. In recent...
16 January 2021 6,923 11 View
Most vaccines contain an infectious pathogen or a part of it, but mRNA vaccines deliver the genetic instructions for our cells to make viral or bacterial proteins themselves. Our immune system...
25 December 2020 3,982 4 View
Hi folks, can anyone provide me with explanations or references regarding why a vaccine would block disease symptoms, but not onward transmission of the pathogen? Leaving aside variations in...
22 December 2020 7,613 4 View
Hi Dose any-one knows about cleaning validation protocols for pathogen-bacteria from fermentor? In industerial size, 1000 lit?
15 December 2020 4,418 3 View
Best methods suitable for isolation and purification of plant pathogenic fungi and bacteria from plant and soil samples....Latest methods....Any special advantages....Procedure to be followed...etc.
28 November 2020 3,365 3 View
Hi, I am trying to screen infected plant proteomes for targets of a secreted pathogen protein. I have purified the pathogen protein using a GST-tag on an agarose resin. I am using the Pierce...
23 November 2020 7,009 3 View
Since the nontuberculous mycobacteria (NTM) are environmental pathogens in which they are found in water, soil, and dust, is there a possibility that NTMs can be isolated in public places or...
06 October 2020 4,878 6 View
I run sequening on a sample and found a pathogenic variant. Then I draw a 3Dprotein model on Swiss-Model for the wild type protein and the mutatnt protein. I don't know how to comment on things...
05 October 2020 579 4 View