Protocol for Transformation of Hansenula polymorpha with Lithium acetate?

24 March 2022 0 1K Report

Hey guys, I want to transformate H. polymorpha with lithium acetate. I'm searching for an extended protocol.

Thank you in advance

Badges
Science method

More Adlen Adil's questions See All

What is the simplest way to implement data encryption for Arduino project that communicate through Bluetooth ?

I have developed a project based on Arduino that communicates through Bluetooth with Android application, I want to enhance its privacy through encryption so that data should be prevented up to...

07 January 2021 7,933 5 View

How to configure wifi password and SSID on NodeMCU ESP8266 through wifi direct instead on hardcoded credentials?

While working on IoT projects I mostly use NodeMCU ESP8266 firmware for IoT Implementation. For coding, I use Arduino IDE in which for connectivity with wifi router we define hardcoded variable...

07 January 2021 6,688 3 View

What criteria should be followed in designing schools?

criteria should be followed in designing schools

31 December 2020 682 0 View

Could anyone suggest the profiling packages in python which similar to profvis package in R?

I am currently working on the benchmarking of the tools and programs developed for handling biological data, I have used R-programming wherein I used Profvis for the R based tools. However, there...

15 December 2020 5,764 3 View

How to plot a Slope gardient Map of Bathymetry?

In order to find out the area/location prone to submarine landslide, plot of slope gradient map of Bathymetry is very helpful. Guide me, how to plot of slope gradient map (in detail/steps) of...

05 November 2020 8,144 4 View

Anyone who wants to work or currently working on federating learning(Distributed Machine Learning)?

I am a Masters Student of Computer Science and Technology at Dalian University of Technology, currently working on fairness/uniformity in highly heterogeneous network of federated learning.

27 October 2020 5,661 3 View

Can you please tell me which problems ,in general, the data mining can solve?

Data mining is a process of discovering patterns in large data sets involving methods at the intersection of machine learning, statistics, and database systems.

26 October 2020 4,364 5 View

What are suitable databases for IoT?

For Internet of things (IoT) which databases are suitable or desigining. I want to know the details of about it.

26 October 2020 2,499 15 View

What are the advantages of a computer network ?

Computer network is a group of computers that use a set of common communication protocols over digital interconnections for the purpose of sharing resources located on or provided by the network...

05 October 2020 8,105 25 View

Homemade liquid fertilizer, How to make it?

Dear Homemade liquid fertilizer derived from manure or organic waste, how we can make it for laboratory experiments. Is there any scientific method/ or papers to produce this product (with...

22 September 2020 3,635 8 View

How to perform statistical analysis of coefficients extracted from transform techniques?

I am studying the potential of DCT and FFT coefficients extracted from a signal to classify the different objects that emit the signal. I applied the Shapiro-Wilk to confirm that data was not...

01 March 2021 8,079 2 View

How to delaminate copper in water-based anodes?

Hello everyone, I'm looking for a way to delaminate copper current collector of water-based anodes in Li-ion batteries without damaging the graphite film. I tried different materials such as HNO3...

24 February 2021 3,785 3 View

Covid 19 Pandemic ' impact on digital transformation?

Covid 19 Pandemic and digital transformation

22 February 2021 3,351 5 View

Where can the fault diagnosis data set of lithium battery be found?

Most of the data sets on the network only contain good information about the voltage and internal resistance of lithium batteries. The data of faulty battery is missing. Who can help me, I will be...

21 February 2021 8,659 1 View

Not able to charge-discharge Li-ion coin cell at higher C rate ?

I am working on cathode material. I have made different Li-ion coin cell against Li metal using commercial available cathode materials such as LCO,LMO, LFP and NMC and also with the lab...

18 February 2021 1,450 3 View

No title

Hello. I ask for help - I need to get lithium tetraborate (soluble salt) from lithium carbonate and boric acid. In literature, everything seems to be simple - mix, ignite and - it's done. But it...

16 February 2021 9,523 2 View

What are the best cations for preparing competent cells, Ca2+ or Mg2+?

I have been reading various articles and most state that treatment with CaCl2 produces higher efficiency of transformation than MgCl2. Can anyone explain why Ca2+ is better than Mg2+ at...

16 February 2021 8,519 5 View

Is the logarithmic transformation a necessary step in time series analysis ?

Hello, I'm checking if the log-tranformation of time series data is a necessary step; We all know the aim of such transformation, but in every study (in time series analysis) we found the authors...

15 February 2021 5,655 14 View

How much amount of DNA (ligation mix) should I use for transformation?

Hi Fellow Scientists! I am trying to clone my 300 bp insert into a 6 kb vector. I run a few different ligation reactions using (1:3 vector: insert molar ratios) 10, 20,30, and 40 ng/ul vector...

15 February 2021 9,563 6 View

How to produce huge amount of protoplasts from Fusarium graminearum?

Hi, all I am using some protocol for protoplast production from Fusarium graminearum (Fg), but I need them in a huge amount. Sometimes the protocol worked well but most of the time failed....

14 February 2021 2,286 4 View