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Questions related from Aidan Cowan
Hello! I am interested in determining whether a poly-unsaturated fatty acid I have engineered E. coli to produce is being incorporated into the membrane of the cells or if it is simply a free...
22 February 2021 7,830 2 View
I am designing an antibody to detect the protein LynA in western blot. Right now I have 8 potential antibodies (SCFVs) that may or may not bind by western blot. I have an apparatus that lets me...
29 November 2018 6,061 1 View
Using a scfv phage library and doing 4 rounds of biopanning against tumor antigen I infected TG1 cells with the phage from the fourth round of biopanning. Infected cells should then contain the...
12 February 2018 5,911 13 View
After 4 rounds of biopanning against a tumor antigen (biotinylated and bound to streptavadin beads) elisas done with scfv that leaked into the culture supernatant show no signals. Western blot...
17 January 2018 4,006 9 View
