I am looking for a protocol for measuring the Kd value of receptor-ligand binding in RPE cells. I have Alexa fluor labeled ligand. It would be beneficial if someone, please share any protocol or reference with me.
I am trying to write a structured literature review on the above question, my topic is MS/EXERCISE.I am not sure that my question is correct or not ,should i make changes in my question?
06 June 2023 9,930 6 View
I am working on SAEW (Slightly acidic electrolysed water) on B.cinerea. But the mode of activity was researched usually on bacteria. In acidic electrolysed water it is considered that the chlorine...
08 January 2020 5,505 5 View
The main concern is to measure the Government performance after the implementation of the GePG system in collecting its monies electronically from the public. And it has been adopted as per the...
11 December 2019 379 2 View
I am performing an experiment in which I am taking 35ml 100% ethanol in a small glass tank. I want to reduce this 100% ethanol concentration by a time-dependent chemical reaction. which reaction I...
01 January 1970 1,010 2 View
I'm guessing it's because the ligand experiences too much electron repulsion or proton repulsion from the chromium to insert them close to the 3d-orbitals which are close to the metal nucleus. Is...
03 August 2024 1,369 1 View
why don't d-orbitals split themselves because of themselves without the presence of ligands? Electrons are indistinguishable. Why wouldn't it be more correct that protons from a ligand split the...
03 August 2024 3,588 3 View
Hello,, The metal complex ligand appears incomplete in the screen of the discovery studio visualizer
30 July 2024 3,353 1 View
When I run autogrid4 it says: autogrid4: ERROR: Unknown receptor type: "Cr" -- Add parameters for it to the parameter library first! Look forward to your reply.Thank you so much!
29 July 2024 486 0 View
Good day, I am a student trying to work on Autodock for a project regarding Ligand-DNA interaction so i am quite new to molecular docking. i have followed tutorials and did all the steps...
28 July 2024 2,136 4 View
I am performing fluorescence experiments using a ligand to detect metal ions. I want to determine the Lowest Detection Limit (LOD) using the formula LOD = 3σ / K. However, I'm uncertain about...
19 July 2024 1,084 1 View
I have been able to assign the CHARMM forcefield to my ligand i.e. acetyl coenzyme A but not the amber forcefield. I want to run amber forcefield and the parameterization of ligand is failing. I...
15 July 2024 7,323 1 View
Hi! I am currently working on a protein where I have selected residues within 10A of its bound ligand in pymol. However, I can not find a way to print out what residues are contained in that group...
15 July 2024 1,726 1 View
Hello! everyone, I am trying to study in silico Protein-Protein and Protein-RNA interactions. Now, is there any tool with which I can identify the interacting amino acid residues or the...
14 July 2024 950 2 View
During the preparation of a ligand molecule in autodock or openbabel Cl atom is not showing in the .pdbqt file whereas it was present in the pdb and sdf file. Due to this Cl atom is not showing in...
13 July 2024 3,486 6 View