Is is good? What reagent is better?
what reagent is better then...?
I do reductive alkylation at up to 15 mg/ml without problems using dimethylamine borane and formaldehyde.
It depends on the behavior of your protein anyway.
I am working on Immunoglobulin, DTT or 2-ME was used as reducing reagent and Iodoacetamide was for alkylation. I am not going use formaldehyde due to keep other protein's bioactivity. Thanks.
If yes, what concentration can be used?
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