Hi all - I apologize if this has been covered before.

What can I expect from a tumor grown from B16-F10 cells in a C57B6/J mouse?

30 days after subcutaneous injection of 10^6 cells, I harvested a massive growth from the abdomen that I assume was a metastasis. However it was WAY bigger than the subcutaneous growth. It is also very soft/mushy.

Is that normal? Do these cells result in very soft tumors usually? The subcutaneous portion was much firmer.

Could it be that they grew too fast and the whole thing became necrotic? Unfortunately the mouse died the morning of the planned sacrifice, so I was unable to properly perfuse and harvest the way I wanted to.

Thank you!