Hi there,

Protease are specific to protein/peptide substrate as they cleave peptide bond and there is no peptide bond in glycolipids. You'd better try glucosidase or lipase.

Thanks Dominique for your response. Appreciated! However, enzyme preparations are usually not pure. What I was asking is if they could also check for other activities as well.

Well if you are interested in contamlnant activities in protease preps then go ahead: only the test will actually tell you.

I have attached a PDF of an enzyme (Olexa) newly developed by Novozymes. I have worked with the enzyme. You might find it interesting to work with the enzyme in your research.

Well thanks, but I'm not into this field at all.

A well purified pro tease always acts on protein moieties and their derivatives. I have not come across a pure proteolytic enzyme that work on other form of substrates that are not protein in nature. However, i will make trials on your suggestion but i am yet to be convinced that  enzymes are not always pure.

Thanks Uzoamaka! It is likely that the enzymes prepared in the laboratory are purer compared to commercial enzyme preparations. I have always worked with commercial enzyme preparations. I have attached a link of the paper we published recently where commercial enzyme preparations were used in the study. Please see Table 2 of the published article and the explanations of the results in the text. Hope you saw the article I attached earlier on the Olexa enzyme (a protease) prepared commercially by Novozymes enzyme company.

https://www.researchgate.net/publication/291419686_Effect_of_malting_time_mashing_temperature_and_added_commercial_enzymes_on_extract_recovery_from_a_Nigerian_malted_yellow_sorghum_variety?_iepl%5BviewId%5D=W2bwWc0d1VVUlPKcROb0EzUM&_iepl%5BprofilePublicationItemVariant%5D=default&_iepl%5Bcontexts%5D%5B0%5D=prfpi&_iepl%5BtargetEntityId%5D=PB%3A291419686&_iepl%5BinteractionType%5D=publicationTitle

Article Effect of malting time, mashing temperature and added commer...