Primers for "OneStep PLUS qMethyl™ PCR Kit"?

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What are the key considerations for selecting the temperature in the thermal regeneration process of adsorbents?

I seek to understand the theoretical basis for selecting the optimal temperature for the thermal regeneration process of exhausted adsorbents, such as activated carbon loaded with dye molecules....

28 June 2024 1,867 3 View

Do I need to autoclave 2X CTAB and Wash buffer?

I am using CTAB method (Naser M. Salameh et al., 2014) for fungal DNA isolation and purification. During reagents preparation I am not sure weather I should autoclave 2X CTAB and Wash buffer. So...

09 June 2024 8,470 2 View

What is the Weight matrix in CNN?

How can we choose the weight matrix in the Convolutional Neural Network? And how this matrix is related to the kernels used in the algorithm?

01 June 2024 2,150 2 View

How can I get the code of openseespy of 3d structures for ida analysis?

I need a code of opensees python for 3d structure for IDA analysis please introduce a good source for this.

30 May 2024 8,926 3 View

Can anyone interpret why the absorbance in alpha amylase assay get increased with increased standard (inhibitor) concentration?

Can anyone interpret why the absorbance (DNS assay method) in alpha amylase assay get increased with increased standard (inhibitor) concentration? it should be decreased with increased standard or...

26 May 2024 4,753 2 View

Could the small transmembrane domain of the recombination protein from the virus be the reason for the lack of expression in E. coli?

I have a protein that is 117 amino acids long, with a 21 amino acid transmembrane domain at the C-terminus. I've cloned this protein into the pET28 expression vector in BL21, but am not seeing any...

20 May 2024 8,161 2 View

GRTensorII Package for Maple?

Please, if anyone has the GRTensorII (not GRTensorIII) algebra package for Maple send me. I tried to get it but I couldn't.

19 March 2024 8,067 0 View

Why get verious result in SVM model , when we shuffle dataset?

require scientific analystis

15 February 2024 1,488 0 View

What is the role of glacial acetic acid in the ring closure of Schiff bases?

06 February 2024 7,842 1 View

I need any data about the influence of avian influenza H9 on broiler and layer performance?

hi all I need any data about the influence of avian influenza H9 on broiler and layer performance, I mean the infunece on egg production, immune response, body weight gain, feed conversion rate,...

13 January 2024 4,360 0 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Anyone having idea about VN primer for miRNA primer design ?

How to design VN primer to attach with universal reverse primer

05 August 2024 2,116 3 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View

I'm optimizing a tetra-ARMS PCR protocol with amplicon sizes: 165bp, 123bp and 93bp. Why, in the gel, only 165bp is visible while I'm expecting all 3?

It's an end-point PCR protocol. I'm using 1.5% agarose gel with SyBR Safe dye and TBE as a running buffer, visualization on BioRad XR+ system. I was primarily thinking of primer efficiency,...

01 August 2024 4,673 4 View

PCR showing no bands - Master mix and primers didn't mix?

Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...

31 July 2024 2,406 6 View

Dimensions of an MJ Research 96-well alpha module?

Can anyone with an MJ research / BioRad PCR machine from ~2010 or earlier tell me the external measurements (LxWxH) of the removable standard PCR alpha modules that can be removed from a PTC200 or...

30 July 2024 2,867 0 View

How to retain amplicon yield after Ampure bead cleanup, following a 2-stage PCR protocol?

Hello all, I have been trying to follow a 2-stage PCR protocol used to amplify barcodes of a large yeast library, as per Nyugen et al. (2022) -...

30 July 2024 841 2 View