Hi Anusha, hope you are doing great and staying safe. Looking at the bamboo images above (Test Method 3), I am really impressed and curious. I am a Ugandan working on a similar project, I am tasked with developing protocols for micropropagation of 3 bamboo varieties (Dendrocalamus giganteus (Giant Bamboo), Oxytenanthera abyssinica (African low land bamboo) and Bambusa vulgaris (Golden/yellow/common). I have however encountered a number of challenges but majorly contamination (fungal and bacterial) of initiated explants and browning of successfully regenerated bamboo shoots (Picture attached). Please advise how I can solve these problems, thanks a lot in advance. Tuhaise Samuel
PS;
I am currently using this media composition for initiation and micropropagation [MS + BAP (5mg/L) + 2, 4 D (1 mg/L) + Vitamin C (5mg/L) + TDZ (0.5mg/L)]
Do you use antibiotic in media?
How do you maintain your mother gardens (do you spray with a bactericide and how regularly?)
Browning can be effective control by frequent subculturing. For contamination control, you may optimize the mercuric chloride concentration.
You can reduce phenol content in addition to subculturing by adding Polyvinylpyrrolidone (PVP) or ascorbic acid in medium culture. Of course, images clearly show that the leaves of the plants became yellow, and this can be attributed to the inability to control phenol and inappropriate plant medium. So I suggest using several culture media or reducing macro salts, make the optimize conditions for this plant.
Finally, I recommend the study of the attached article.
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