Hello

Using fast freezing (power freeze)

- The desired weight of the desired Joonail sample is 50-100 grams,

Depends on the fish sample

- Place the sample in a polyamide bag

- Avoid stacking juveniles

Ratio of biochemical changes in tissue samples

- The first month 1.7 percent

- The second month 9.1 percent

- Third month 2.2 percent

- 4.3% in the fourth month

- The fifth month 1.6%

- Sixth month 8.6%

- The best time for molecular work is up to the second month

Be very careful about these points so that you do not make mistakes in molecular work

However, this is my advice

Good luck

Hi

I am not sure you are right about -20C for RNA extraction. As far as I know, it should be -80 for RNA extraction. Otherwise, you can use the solution RNA-Later. This preserves the samples for RNA ectraction at room temerature and then at -20

Dear Nava,

For RNA isolation, take the sample in RNA Later (ratio 1:5: to 1:10, 50-100 microgram sample in 500-1000 micro litre RNA Later, sample must be properly dipped into RNA LATER). Cut the sample in small pieces for better penetration of RNA Later. If you wish to isolate RNA within 5-7 days, keep the sample in normal RT. Or if you wish to do later, keep the samples in 4 degree freezer for overnight followed by storage at -80 degree C for preservation.

Thanks

Dear all, Thank you very much for your responses Afshin Mahmoodzadeh Seyyed Morteza Hoseini Prasanta Jana

for DNA extraction you can keep in 70% alcohol, For both DNA and RNA extraction (If you are using same tissue), Keep in Trizol regent (a commercial mix of guanidium thiocyanate and phenol)

Dear Nava,

You can use RNA later or Trizol or Ribozol (Based on the availability) for preserving the sample. However, you have to ensure that the sample is properly dipped in the solution and preferably keep in -80 if it is for long term storage.

70% ethanol is enough for preserving samples for DNA extraction, but not for RNA extraction. If same sample is going to use the study, then first one will be better

Shamna Nazeemashahul Tincy Varghese Thank you for your responses.