How to clone pcDNA vector in SH-SY5Y cell line?

Dear all, How I can clone two genes in pcDNA vector, simultaneously (one of them is target gene with 500 bp length and one of them is GFP). Also, how we can transfect the vector into SH-SY5Y cell...

12 November 2020 6,122 3 View

I am looking for a text book on quantum theory which is suitable for mathematicians. Does anyone have a suggestion (instead of B C Hall's one)?

I mean a book which is written in math texts style. I saw Hall's book but I am looking for a book whuch has more details.

30 October 2020 1,636 6 View

How to simulate MSE walls for dynamic FE analyses?

Hi colleagues, Because of the complexity of modelling geogrid layers and their interfaces which can cause convergence problem in FE analyses, I have seen in practice they model the whole MSE wall...

14 August 2020 4,649 3 View

How I can see contact stress in my analysis result in abaqus ?

I want to report contact stress in my result, is that cpress (contact pressure) ? what is the different between contact stress and contact pressure? !

27 July 2020 3,767 3 View

Is it possible to simulate tip-sample interaction during Scanning Probe Microscopy in Abaqus? and if not which software do you recommend?

I want to simulate this interaction in Abaqus but I heard it may be possible only in Comsol, considering the samples are measured in Nano scale, thanks

01 April 2020 8,313 3 View

Does industrial scale Hydrometallurgical purification of Silicon exist?

There are several papers describing different hydro-metallurgical purification routes of Metallurgical Si to Solar Si through alloying, doping, nano sized grinding,... and finally leaching in...

28 March 2020 2,201 3 View

Why TiO2 powder earnd from sulfate process turns yellow after anealing?

Tio2 powder is earned after sulfate process by solving illmanite in the sulfuric acid and a purity of up 95% of TiO2 is gained . why the color of Tio2 turns yellow after 2h annealing in 900 oC...

07 February 2020 5,769 3 View

How to fine grind material in a narrow band particle size distribution?

Does anybody have ideas to mill Silica powder to micron size particles to a narrow particle size distribution (PSD)? For example grinding with planetary ball mill makes a wide PSD which is not...

19 December 2019 5,431 2 View

Hi all, could you please recommend a good review paper on the scheduling of renewable energy resources (RESs) in microgrids?

I would be very thankful if anyone could recommend some good review papers about the scheduling or economic dispatch of RESs in the microgrids. The assessment of the greenhouse gasses in the...

05 December 2019 6,335 3 View

Diffused dye front on SDS-PAGE, any suggestions on what the problem might be?

I ran these 15% gels for 90 minutes under 200 volts ( gel ran too slow), ladder looked very condense on the top and the dye front was somehow diffused at the bottom of the gel. I am sure about the...

16 April 2019 6,092 5 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

Which is suitable for use with Python? MySQL or SQL Server?

Which is suitable for use with Python? MySQL or SQL Server? What is your suggestion?

01 March 2021 3,422 3 View