i am working on growth pf probiotics in different prebiotics. when i grow them in media devoid of any other normal sugar and giving them only my prebiotic sugars. they have a very slower growth rate,with not much pH drop. so how can i claim,,prebiotics are better supporter for probiotics?
Hello Kishoree,
"Prebiotics are good for probiotics growth."
You say so...
Prebiotics may be good for some bacteria present in intestinal microbiota. So they promote the growth of some bacteria (such as bifidobacteria and lactobacilli) in a competition environment. This environment is dramatically the opposite of "pure" culture...
Kind regards.
Hi Kumaree
You can mix between prebiotic and probiotic and add to 100 ml of skim milk grow at 37 C for 24 after this . You will count of probiotic bacteria than with control sample without prebiotic.
If you do this, you'll count bacteria which don't have lactate deshydrogenase (or don't express well their gene)... not for probiotics needing prebiotics for growth...
Regards
Dear Ms. Kumaree,
Growing probiotics in the presence of prebiotics, and then measuring the amount of reducing sugars produced in the media (minus the control), might give us an idea of prebiotic utilization by probiotic bacteria. Probiotics should theoretically decompose and digest the prebiotics and converted it into (technically) glucose, the simplest sugar, considering that other factors of prebiotic breakdown are taken into consideration (like breakdown of prebiotics by acids produced by the probiotic). Having said that, the acid related breakdown of prebiotics by probiotics can most certainly be also a mechanism of utilization.
Regards,
Pallab
Prebiotic are good for all bacteria and so for probiotics but also for other species. Prebiotics provide "intact" carbon source in the colon and are known to promote ecological biodiversity. They do not promote the growth of only one or two species even less they do not promote the growth of only some probiotics. It exist thousand bacteria that can use prebiotics better than may does probiotics...
Finally providing prebiotics in vitro culture would results in the same results as providing glucose (or some others monosaccharides) as the metabolic crossroads in cell energy production is pyruvate which is mainly produce from monosaccharides.
Best regards
http://onlinelibrary.wiley.com/doi/10.1111/1751-7915.12049/full
Dear Mr Cyril,
may be the results will be same as normal sugar. but my focus is how does it affect probiotics efficiency in delivering their duty towards our gut. will it be of any help to probiotics and indirectly helping us too?
When you administer probiotics to someone, you give like 108 or 109 bacteria (per pills) which will be delivered in a environnement containing between 1011 and 1012 bacteria per grams (in the colon), which constitute 1014 bacteria in total.
If you add prebiotics in you pills, actually only few of them would used by your probiotics, because prebiotics would be mainly consumed by others bacteria (in much higher proportion)...
Maybe it could help probiotics after they colonized the environment, but recent literature reports more helps in biodiversity than in growth of one species.
Synbiotics (combinations of probiotics and prebiotics) have been tried in simulated environments with substantial pH drop. So your thesis of not having significant pH drop may be due to the culture environment, or possibly due to the age and condition of the culture.
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