Hi everyone,
We are going to perform some immunohistochemistry using c-fos antibody to look at the expression patterns of fos protein in different brain regions. I know that most people postfixate their brains in PFA for 24 h but I read papers that saying brains should not be kept in postfixation more than 1 hour for c-fos staining and also half-strong fixative should be use for that purpose.
So how long should I keep my brains in postfixation and how strong should it be?
Hi, we also had success by doing 4% PFA cardiac perfusion then post-fixation in PFA% at 4 degrees for 48 hours then sucrose until they sink to the bottom of the vial. We rinsed the floating brain slides with PBS 1x for 4x 10 min and then left them in PBS overnight prior to c-fos staining
- Badges
- Science topic