Positive IC50 values are commonly recorded. Is it possible to get negative IC50 values?

08 August 2015 1 2K Report

is it possible to get negative value lC50 Antioxidant assay( plant and mushroom)??????

please add some more information.

IC50 value is always positive because you determine a value from a positive control compared to incubated or exposed cells.

if your assay gives you a negative value then you will have a problem in your assay.

either your controls are not growing or your medium is giving a too high background signal.

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