I am now doing a native polyacrylamide in-gel assay of malate dehydrogenase and alcohol dehydrogenase at 8% gel but it did not give very good results. The staining recipe was adapted from the paper Ayala et al. (1972). Please help me and provide me with a staining recipe for both enzymes.
Our article published in peer-reviewed Journal "Communicative & Integrative Biology". A few major points discussed in the paper:
(1) Brain is not the source of consciousness.
(2) Consciousness is ubiquitous in all living organisms, starting from bacteria to human beings.
(3) The individual cells in the multicellular organisms are also individually cognitive entities.
(4) Proposals like “artificial life”, “artificial intelligence”, “sentient machines” and so on are only fairytales because no designer can produce an artifact with the properties like internal teleology (Naturzweck) and formative force (bildende Kraft).
(5) The material origin of life and objective evolution are only misconceptions that biologists must overcome.
http://www.tandfonline.com/doi/full/10.1080/19420889.2015.1085138
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