Selective sweeps generally only affect genetic variation at very closely linked loci, but the stronger the selection, the greater the extent of the effect. So it is usually evidenced by extensive sequence data, say along most of the length of a chromosome. I think you would need unusually extensive and dense sampling of isozyme loci to see a convincing reduction in heterozygosity in a chromosomal region. Allozymes don't reveal synonymous substitution and loci are highly heterogeneous in levels of heterozygosity. So all in all, I don't these data are suited to the task.

Thank you very much Prof. Wallis.

Hi Sanjay

I totally agree with Prof Wallis. But if you still want to test your data, you can use Fdist2 (http://popgen.net/soft/lositan/       I can not find the original website for the Fdist2 software, but Lositan uses it) or Bayescan (http://cmpg.unibe.ch/software/BayeScan/). You can also use Arlequin (http://cmpg.unibe.ch/software/arlequin35/) for a method similar to Fdist2 with a hierarchical genetic structure.

Hope this helps

Thank you Dr. Goicoechea. I'll try my best

for that kind of data, one possibility is to check the number of rare alleles (Chakraborty, Ewens...). I think you can do it with Arelquin, not sure.

But I agree, you are much better off with sequence data (or at least haplotype sof SNPs)

best