How to run ModelTest + PartitionFinder on IQTree? What am I doing wrong, please?

In order to discover the best evolutionary model and partitioning scheme for Bayesian analysis, I am trying to run the command line iqtree -s aln_seqs.fas -p aln_seqs.nex -m MF+MERGE -mset...

17 November 2020 6,403 2 View

How can I discover what is the problem with my PCR?

I am trying to perform a SSP-PCR of HLA genes. The reaction is optmized as it has worked with my controls. But when I try to amplify my cancer samples the reaction does not work. Some samples do...

12 May 2019 2,795 4 View

How to calculate ABSI by excel?

13 March 2019 3,774 3 View

Does anybody have Kalign for Windows?

Hello, everyone. I'd like to know if anybody has an executable file of Kalign tool for Windows, since it is, nativally, for Unix. Thanks!

23 October 2018 3,626 4 View

Sample preparation_Luminex CNS?

15 May 2018 3,313 2 View

Can anyone help me to interpret the results of a back titration in a virus neutralization experiment?

I have no experience in this field and I really need to know if the results of my back titration are between 30 and 300 TCID50. How can I calculate the TCID50 that I obtained as a result of the...

29 January 2017 2,826 2 View

Why my colony PCR is positive but when the clone is sequenced there isn't an insert?

I am trying to cloning a protein in the pPICZalfaA vector, with the restriction enzyme SalI and XhoI, the colony PCR (with the specific forward primer for insert and reverse primer of vector) is...

16 February 2014 7,944 13 View

How can I purify proteins from the SDS-PAGE gel without blotting? Can I use this protein to do Edman's degradation?

Is there any protocol or kit to do this?

22 November 2012 389 13 View

Logistical regression using SPSS for a dichotomous dependent variable, with an independent variable of 1, 2, 5 and 10 seconds?

I have an experiment with 28 participants The independent variable is the times 1, 2, 5, and 10 used as the cross hairs during a task These 4 times were tested using 4 different blocks, each...

03 March 2021 9,692 2 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

How to choose ANN number of hidden layers and nodes?

Hello, Could you please share any interesting research explaining how to choose the number of hidden layers and nodes per layer in case of regression problems using ANN? Thank you, any help would...

01 March 2021 6,200 3 View

Can anyone provide the Support vector regression code structure of LIBSVM MATLAB or python ?

Please, if Gaussian Parameter optimization is possible in this code structure

28 February 2021 278 3 View

How could I take into consideration the weights of observation in a system of seemingly unrelated regressions (SUR) in rstudio?

I build a model of 4 regression equations (probit and OLS) in R studio. I need to take the weights of observations into consideration. While it is simple to do so for every regression equation,...

27 February 2021 1,233 1 View

VIF very high, but correlation is low and coefficient is significant. Which one whould I choose?

Hello. I have a question whether if my coefficients (statistically significant) would be valid when VIFs are really high. In my model, there are independent variables(IV), moderating variables...

26 February 2021 956 13 View

Treating Intermediate Variable in Linear Regression Analysis in SPSS?

While performing the Linear regression analysis in SPSS version 26.0.0. my intermediate/mediation variable goes to the excluded section. Please share your valuable suggestion "How to restrain the...

25 February 2021 4,591 5 View

Cox regression baseline hazard?

Dear colleagues, I would like to make a mathematical equation of Cox regression model, however I am stuck with baseline hazard. I clearly understand interpretation of coefficients, but I can't...

24 February 2021 6,207 3 View

How to run raise regression in R?

if anybody know how to run raise regression in r, and raise parameter selection, please help me

24 February 2021 8,199 1 View

Can anyone suggest me the best way for Regression analysis of Accidents?

Dear Altruist, I want to make a regression analysis based on accident data. But I am not finding the necessary information about what type of regression is more applicable for analysis accident...

23 February 2021 9,956 3 View