I have analysed genetic diversity of apple cultivars. I have taken sample of same cultivars from different locations. Using ISSR and SSR markers I have found that almost 60 % molecular variance...
09 October 2014 1,465 12 View
I have sterilized the working lab, autoclaved the plastic wares and have also changed the reagents like buffer, MgCl2, DNTP, Taq, water etc. I am generally working in Laminar flow hood.
06 July 2014 1,723 8 View
Reg. Hplc standard curve calculation. Please tell me how to calculate limit of detection, limit of quantification and signal to noise ratio. Please also explain what is the relation of these...
04 May 2014 1,549 75 View
I am using a C 18 Column to estimate flavonoids with mobile phase (50:50) as Methanol and 0.5% ortho phosphoric acid. There is also a shift in pressure.
03 April 2014 7,727 25 View
Sample species is from Rosiaceae
31 December 2011 2,214 18 View
I'm very interested in biotechnology and medical research and I'd like to meet a professional here and talk about it together
28 July 2024 3,798 4 View
List down the various factors responsible and how does this effect the overall biogas production when it is related to Hydraulic retention time of the digester based on the feedstock?
27 July 2024 4,800 4 View
I am pleased to announce the CUSABIO Biotechnology Scholarships for Students 2024! This scholarship aims to support outstanding students pursuing studies in the field of biotechnology. Here are...
22 July 2024 5,790 1 View
I was wondering if it is possible to form a permanent open "ssDNA bubble" similar to a transcription bubble (>13 nucleotides) within E. coli. These criteria are important: 1. Open ssDNA...
07 July 2024 8,275 5 View
Give in detail with available literature and website links for submissions
27 June 2024 9,753 1 View
Hey everyone! I'm having difficulty marking the C4 complement factor in Western Blotting. Has anyone had contact with this antibody or made it in-house? I have already used Anti-C4β (D-12)...
17 June 2024 6,124 1 View
Generally for bacterial DNA isolation we use a fresh culture of 2-5 days old. But if we use an older culture say 10-15 days old will it have any impact on the DNA content and the DNA isolation...
13 May 2024 3,061 11 View
I was curious as to why modern soybean half-seed protocols use 1/10x strength B5 salts during the co-cultivation step rather than full strength B5 salts? Also, are the B5 vitamins added at full...
07 May 2024 3,924 0 View
I am following the standard method which is mentioned in Tomotake Kanki et al., 2009 research paper Monitoring mitophagy in yeast: The Om45-GFP processing assay . I am growing the cells in SD-HIS...
04 May 2024 6,463 0 View
How are these exploited in biotechnological applications?
07 April 2024 566 5 View
