Praveen Dhyani

6 Questions 5 Answers 0 Followers

Questions related from Praveen Dhyani

Can there be molecular variance within and among apple cultivars from different locations?

I have analysed genetic diversity of apple cultivars. I have taken sample of same cultivars from different locations. Using ISSR and SSR markers I have found that almost 60 % molecular variance...

10 October 2014 1,449 12 View

How can I get rid of the contamination of negative control in PCR? I am using SSR marker.

I have sterilized the working lab, autoclaved the plastic wares and have also changed the reagents like buffer, MgCl2, DNTP, Taq, water etc. I am generally working in Laminar flow hood.

07 July 2014 1,697 8 View

How to calculate limit of detection, limit of quantification and signal to noise ratio?

Reg. Hplc standard curve calculation. Please tell me how to calculate limit of detection, limit of quantification and signal to noise ratio. Please also explain what is the relation of these...

05 May 2014 1,378 75 View

I have observed a shift in RT in my HPLC. Please advise.

I am using a C 18 Column to estimate flavonoids with mobile phase (50:50) as Methanol and 0.5% ortho phosphoric acid. There is also a shift in pressure.

04 April 2014 7,701 25 View

Is it possible to detect amino acids, sugars and vitamins with help of HPLC esp HPLC having UV detector?

Sample species is from Rosiaceae

01 January 2012 2,194 18 View

Plz tell me, How do I stain the AFLP gel?

I have tried silver staining but that did not worked very well..

10 October 2011 2,376 0 View