Please, is there suitable solvent to fractionate fatty esters into medium molecular weight fatty esters and high molecular weight fatty esters?

I want to separate a complex mixture of esters into medium and high molecular weight esters using solvent extraction.

11 October 2020 9,891 2 View

Is multicollinearity a problem in regression model with big data?

I asked this because according to Gujarati (1995), one of the remedial measures to mitigate the problem of multicollinearity is to increase the sample size. Thus, can we say that multicollinearity...

17 July 2020 3,786 7 View

What are the calculations involved High hydrostatic pressure machine?

I need calculation in the design of High hydrostatic pressure machine

04 June 2020 5,515 4 View

What are the interractions of Classicism and Romanticism in the society and how do these lead to a change in the society?

Classicism is a philosophy of conservatism, imitation of the famous experts and mentorship whereas Romanticism is a philosophy of freedom, intuition, quest for adventure and realization of...

24 February 2020 9,091 9 View

Studying boundary value problem, is it reasonable to think of first order ODE?

I am very familiar with problems of the form y'(x) = f(r; x, y), y(a)=y0, y(b)=y1 where r is a parameter to be estimated using the boundary values. But apart from this situation, has anyone...

23 October 2019 2,864 3 View

How does oil swelling as a result of CO2 dissolved in crude oil increases the oil recovery?

Enhanced oil recovery

30 August 2019 365 2 View

Why is Patch Dock returning my docking search?

the Patch Dock is giving me trouble regarding the code for my ligand the error message is " Error in receptor molecule input: please specify PDB code or upload file " My ligand is microRNA. and it...

19 June 2019 2,638 2 View

How do you cope when you have a major paper presentation and your paper is not ready?

Many times in my experience as an academic, I arrive at a conference venue with the nagging feeling that my paper is not yet what it should be. Lead paper presenters have sometimes confessed to...

26 December 2018 5,499 5 View

What database can i find the expressions of colorectum as a tissue ?

Dear all, I want to extract the genes related to colorectum but the database TIGER only showed the tissue view of colon. please suggest any database that can give the ESTs or expression profile of...

29 April 2018 5,910 4 View

Which online target prediction tool is best for predicting miRNA targets considering a friendly interface ?

I need to identify some targets of a number of miRNAs but i need suggestions on the best target prediction tool or a combination majorly for humans. Thanks

29 April 2018 1,323 2 View

Hello, how can i find a primer to identify Bacillus Sp. with PCR? Can someone help me with this?

I am looking for PCR primers for bacillus Sp.

15 February 2021 2,552 5 View

the product size is 191bp would it be a problem during conventional pcr?

I have designed a primer for Bacillus subtilis sfp gene. As I have mentioned the size is 191 bp. however I need some suggestions regarding PCR temperatures and cycle length. As the it is smaller...

09 January 2021 8,309 4 View

No title

Hi, I am interested to see the effect of BCG on macrophages (microglia )Therefore , I would like to know the amounts and the routes of administration which is safe in mice and how long is...

20 December 2020 7,166 2 View

How to handle milk clotting Issues during media preparation for Screening of Halotolerant Protease Producing Bacteria (with Skim Milk)?

We want to screen halotolerant protease producing bacteria. Since Skim milk (HiMedia) is available in our lab, therefore, we want to screen extracellular protease by this compound/ supplement. We...

04 December 2020 7,657 6 View

Best way for knockout of gene in Bacillus genome?

Hi everyone! I would like to knockout a gene in the genome of Bacillus. To do that I tried to make a construct that has 1) a backbone, 2) a kanamycin resistance cassette, flanked by 3) the...

24 November 2020 489 1 View

How can i get nonsporulating bacterial bacillus subtillis strain spoIIE without antibiotic growth conditions ?

10 November 2020 7,168 4 View

Can someone help me to understand IGEM 2016 protocol to Bacillus subtilis transformation?

I'm trying to transform Bacillus subtilis to fluorescence, and I will use this IGEM protocol with medium MNGE, but there's a part that I can't understand. It says: "Measure the OD600 of your...

29 October 2020 2,043 1 View

How does the spores of Bacillus anthracis cause the anthrax disease and how can it be weaponized?

Anthrax is a disease caused by aerobic spore-forming Bacillus anthracis. This spore can resist environmental changes and resist biological extremes of heat, pH, chemicals, and other factors; it...

20 October 2020 4,658 8 View

Can I transform Bacillus Subtilus with ligation reaction directly?

Hi All, I want to transform bacillus subtilus with ligation reaction directly. Is that possible? To be more clear, I have a shuttling plasmid between bacillus and e.coli. I know usually we need...

14 October 2020 1,743 1 View

I am not able to find the best working protocol for making electrocompetent cells for bacillus sp. Can anyone help me with this?

I am using a protocol for Bacillus subtilis for which there is a requirement for special electroporation media having sorbitol, mannitol and glycerol. I am using puc18 plasmid (conc...

16 July 2020 3,417 2 View